cmv brainbow 2 1 r Search Results


brainbow construct v2 1  (Addgene inc)
93
Addgene inc brainbow construct v2 1
(A) Homogeneous and non-homogeneous models differ in whether the 2 blastomeres at 2-cell stage have identical tendencies to contribute their descendent cells to inner cell mass (brown) and trophectoderm (blue-grey). (B) Cell lineage tracing was enabled by creation of preimplantation embryos heterozygous for <t>brainbow</t> sequence and Cre, and induction of Cre at 2-cell stage. At blastocyst stage, activated brainbow genes can exhibit either relatively equal (balanced) or biased (unbalanced) distributions in inner cell mass and trophectoderm.
Brainbow Construct V2 1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cmv+brainbow+2+1+r/CMV-Brainbow-2%2E1+R+(Plasmid+%2318723)/bio_rxiv__293134-159-18-21
Average 93 stars, based on 1 article reviews
brainbow construct v2 1 - by Bioz Stars, 2026-09
93/100 stars
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(A) Homogeneous and non-homogeneous models differ in whether the 2 blastomeres at 2-cell stage have identical tendencies to contribute their descendent cells to inner cell mass (brown) and trophectoderm (blue-grey). (B) Cell lineage tracing was enabled by creation of preimplantation embryos heterozygous for brainbow sequence and Cre, and induction of Cre at 2-cell stage. At blastocyst stage, activated brainbow genes can exhibit either relatively equal (balanced) or biased (unbalanced) distributions in inner cell mass and trophectoderm.

Journal: bioRxiv

Article Title: Rainbow-seq: combining cell lineage tracking with single-cell RNA sequencing in preimplantation embryos

doi: 10.1101/293134

Figure Lengend Snippet: (A) Homogeneous and non-homogeneous models differ in whether the 2 blastomeres at 2-cell stage have identical tendencies to contribute their descendent cells to inner cell mass (brown) and trophectoderm (blue-grey). (B) Cell lineage tracing was enabled by creation of preimplantation embryos heterozygous for brainbow sequence and Cre, and induction of Cre at 2-cell stage. At blastocyst stage, activated brainbow genes can exhibit either relatively equal (balanced) or biased (unbalanced) distributions in inner cell mass and trophectoderm.

Article Snippet: Sequencing reads from each cell were aligned to DNA sequences of the four fluorescent protein genes present on brainbow construct v2.1 (Addgene plasmid repository 18723) using STAR aligner.

Techniques: Sequencing

(A) Recombination of brainbow construct was induced in early 2-cell embryos, and images were acquired at 4-, 8-cells and blastocyst stages. GFP: green fluorescent protein; RFP: red fluorescent protein; DIC: differential interference contrast. Scale bar: 15um. Image was acquired by a wild-field microscope containing filters for RFP and GFP. (B) Two representative blastocyst stage embryos imaged with DIC, DAPI, GFP, and RFP channels. (C) Numbers of blastocyst-stage embryos with balanced (orange) and unbalanced (blue) lineage marker expression in the two embryonic poles, with respective counting methods (three lanes). Data from different counting methods are shown in columns, which include requiring at least 1/3 cells (One third) or 1/2 cells (One half) expressing either GFP or RFP, and all the imaged embryos (All).

Journal: bioRxiv

Article Title: Rainbow-seq: combining cell lineage tracking with single-cell RNA sequencing in preimplantation embryos

doi: 10.1101/293134

Figure Lengend Snippet: (A) Recombination of brainbow construct was induced in early 2-cell embryos, and images were acquired at 4-, 8-cells and blastocyst stages. GFP: green fluorescent protein; RFP: red fluorescent protein; DIC: differential interference contrast. Scale bar: 15um. Image was acquired by a wild-field microscope containing filters for RFP and GFP. (B) Two representative blastocyst stage embryos imaged with DIC, DAPI, GFP, and RFP channels. (C) Numbers of blastocyst-stage embryos with balanced (orange) and unbalanced (blue) lineage marker expression in the two embryonic poles, with respective counting methods (three lanes). Data from different counting methods are shown in columns, which include requiring at least 1/3 cells (One third) or 1/2 cells (One half) expressing either GFP or RFP, and all the imaged embryos (All).

Article Snippet: Sequencing reads from each cell were aligned to DNA sequences of the four fluorescent protein genes present on brainbow construct v2.1 (Addgene plasmid repository 18723) using STAR aligner.

Techniques: Construct, Microscopy, Marker, Expressing